Analysis Note

Positive ControlHT-1080 or ConA-treated MD-MB-231 cells

Application

Immunoblotting (10 g/ml)Immunofluorescence (see application references)Immunoprecipitation (see application references)Paraffin Sections (see application references)

General description

Recognizes the ~60 kDa MTI-MMP protein in HT-1080 cells and in ConA-treated MD-MB-231 cells.

This Anti-MT1-MMP (Ab-4) Mouse mAb (113-5B7) is validated for use in Immunoblotting, Immunofluorescence, Immunoprecipitation, Paraffin Sections for the detection of MT1-MMP (Ab-4).

Purified mouse monoclonal antibody. Recognizes the ~60 kDa MTI-MMP protein in overexpressing cells.

Immunogen

a synthetic peptide (CDGNFDTVAMLRGEM) corresponding to amino acids 319-333 in the hemopexin-like domain of human MT1-MMP

Human

Legal Information

Manufactured by Daiichi Fine Chemicals C0., Ltd., Not available for sale in Japan.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Sakakibara, M., et al. 1999. Cancer85, 231-239.Mattei, M.G., et al. 1997. Genomics:40, 168.Okada, A., et al. 1997. J. Cell. Biol.147, 67.Okumura, Y., et al. 1997. FEBS Lett.402, 181.Ueno, H., et al. 1997. Cancer Res.57, 2055.Strongin, A.Y., et al. 1995. J. Biol. Chem.270 5331.Takino, T., et al. 1995. J. Biol. Chem.270, 23013.Cottam, D. W. and Rees, R. C., 1993. Intl J. Oncol.2, 861.Stetler-Stevenson, W. G., et al. 1993. FASEB J.7, 1434.Fridman, R., et al. 1992. J. Biol. Chem.267, 15389.Woessner, J. F., 1991. FASEB J.5, 2145.Liotta, L. A. and Stetler-Stevenson, W. G., 1990. in Sem. Cancer Biol., ed. M. M. Gottesman. Vol. 1(2), 99.Goldberg, G.I., et al. 1989. PNAS86, 8207.

This antibody cross-reacts slightly with human MMP-3. To induce MT1-MMP in MD-MB-231 cells, treat with 1-5 µg/ml ConA for 24-48 h. Antibody should be titrated for optimal results in individual systems.

Packaging

100 µg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Physical form

In 100 mM sodium phosphate buffer, 0.1% BSA, pH 7.0.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Warning

Toxicity: Standard Handling (A)